Selecting and saving PCR primers

To create a primer file using the Alignment editor:

  • Use the mouse to highlight the region to be used as a primer sequence.
  • Right click on the highlighted sequence to display the context menu.
  • Select the Save as subsequence menu item.
  • In the Save as subsequence dialog box:
    • Enter the sequence name and definition line.
    • Select the output file location, using the browse dialog box if desired.
    • Select the Reverse complement option if a reversed primer is required.
    • Use the preview pane to review the output file, then press OK to save the primer file.

Save subsequence